Fig. 6: Combined SIRT1 and HSP90 inhibition causes synergistic/additive cytotoxic effects in OxPhos-DLBCL cell lines: K422, Toledo and Ly4.
A SIRT1 knockdown with shRNA significantly decreases the proliferation of K422, Ly4 and Toledo. Cells were seeded at the concentration of 0.2 × 106 cells/ml and counted for the four consecutive days using Trypan Blue exclusion. Graphs show averaged values ± SDs from a representative of three independent experiments performed in triplicates. Statistical analyses were performed using t test; ** for p < 0.01 and * for p < 0.05. B HSP90 and SIRT1 inhibitors exhibit synergistic/additive effects in OxPhos-DLBCL cell lines K422, Toledo and Ly4. Cell viability was assessed using MTS assay. Graphs show averaged data from three independent experiments ± SDs. CI - combination index. CI values between 0 and 0.9 indicate synergy, CI between 0.900 and 1.100 indicates additivity, and CI ≥ 1.100 indicates antagonism.
