Fig. 4: PSMD2 is a novel binding partner of RACK1 and β-catenin. | Cell Death & Disease

Fig. 4: PSMD2 is a novel binding partner of RACK1 and β-catenin.

From: RACK1 facilitates breast cancer progression by competitively inhibiting the binding of β-catenin to PSMD2 and enhancing the stability of β-catenin

Fig. 4

A Co-immunoprecipitation (Co-IP) analysis of the interaction pattern among RACK1, β-catenin, GSK3β and Anxa2 in MDA-231 and BT549 cells. B Co-IP analysis of the interaction pattern among exogenous RACK1-flag, β-catenin-myc and GSK3β in HEK-293T cells. C Co-IP analysis of the interaction pattern among endogenous RACK1, β-catenin, and GSK3β in gastric AGS cells. D The protein score of the top 5 proteasome components identified in RACK1-interacting proteins. E Representative mass spectra of PSMD2. F Co-IP analysis of the interaction between RACK1 and PSMD2 in MDA-231 cells expressing RACK1-flag. G Co-IP analysis of the interaction pattern among exogenous PSMD2-flag, β-catenin-myc and RACK1 in HEK-293T cells. H Co-IP analysis of the interaction pattern among endogenous RACK1, β-catenin and PSMD2 in MDA-231 and BT549 cells.

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