Fig. 6: Analysis of the neurite network in WT and nHOTAIRM1 KO post-mitotic spMNs. | Cell Death & Disease

Fig. 6: Analysis of the neurite network in WT and nHOTAIRM1 KO post-mitotic spMNs.

From: The long noncoding RNA nHOTAIRM1 is necessary for differentiation and activity of iPSC-derived spinal motor neurons

Fig. 6

A qRT-PCR analysis of genes involved in neurite outgrowth and branching (indicated above each panel) in WT and nHOTAIRM1 KO (KO#2 clone) spMNs (D12). Data (means ± SEM) are expressed in arbitrary units, relative to ATP5O mRNA levels, as internal control. For each target, the expression in WT spMNs was set as 1. N = 3 biological replicates; *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, two-tailed Student’s t test. B Representative acquisition and skeletonization of neurite networks in WT and nHOTAIRM1 KO (KO#1 and KO#2 clones) spMNs (D12). Scale bar units are indicated in each panel. C–G Number of somata (C), number of branches (D), number of junctions (E), total branch length (F), and average branch length (G) per acquisition in WT and nHOTAIRM1 KO (KO#1 and KO#2 clones) spMNs (D12). Horizontal lines in boxplots indicate median values; boxes extend from the 25th to the 75th percentile of each group’s distribution of values; black dots represent outliers. Boxplots represent the value distribution from N = 3 independent biological replicates. In all, 10–20 acquisitions with a size of 212.3 μm × 212.3 μm have been analyzed for each replicate. *P ≤ 0.05, ****P ≤ 0.0001 correspond to one-way ANOVA multiple comparison test.

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