Fig. 3: SAA suppresses α-PD-1 induced anti-tumor cytotoxic immune responses.

A Flow chat of autologous ex-vivo tumor organoids-PBMCs co-culture model. B α-PD-1 (Tislelizumab) was introduced in the co-culture model and the supernatant were collected for LDH measurement (mean with SD, one way ANOVA with Post Hoc analysis). C, D The α-SAA neutralization antibody and rec. SAA1 protein were introduced in the co-culture model, after that the supernatant were collected for LDH measurement (immune-cytotoxic assay) (mean with SD, one way ANOVA with Post Hoc analysis). E α-SAA neutralization antibody and rec. SAA1 protein were introduced in the co-culture model, after 3 days cells were collected for immunophenotyping by flow cytometry. F, G the percentage of CD45⁺/CD3⁺/CD8⁺ CTLs and CD45⁺/CD3⁺/CD8⁺/GrB⁺ CTLs were measured (mean with SD, one way ANOVA with Post Hoc analysis). H Ten lung cancer FFPE samples were enrolled for immunofluorescence imaging, the SAA, CD8, Pan-CK and DAPI were stained. I The correlation between the percentage of CD8⁺ cells and SAA⁺ cells were evaluated (linear regression).