Fig. 5: SAA drives malignant cells secrete type-2 immunity promoting cytokines.
From: SAA suppresses α-PD-1 induced anti-tumor immunity by driving TH2 polarization in lung adenocarcinoma
In the organoids-PBMCs co-culture, PBMCs or organoids culturing model, the OVA (Ovalbumin) were added to induce type 2 immunity. A After 3 days, the supernatant IL-33 concentration was measured in co-culture model (mean with SD, one way ANOVA with Post Hoc analysis). B After 3 days, the supernatant IL-33 concentration was measured in PBMCs culture model (mean with SD, one way ANOVA with Post Hoc analysis). C After 3 days, the supernatant IL-33 concentration was measured in organoids culture model (mean with SD, one way ANOVA with Post Hoc analysis). D, E The α-IL-33 neutralization antibody and rec. SAA1 protein was introduced in the co-culture model, after 3 days the cells were collected for immunophenotyping by flow cytometry (mean with SD, one way ANOVA with Post Hoc analysis).
