Fig. 7: ITIMs/LAIR1 and PTP/SHP2 were required for the nuclear localization of FAK.

A LAIR1–SHP2 and SHP2–FAK interactions in LAIR1OE GL261 cells by co-immunoprecipitation. Normal rabbit IgG antibody was used as control. n = 3. B LAIR1–SHP2 (a) and SHP2–FAK (b) docking result. LAIR1 was displayed in red, SHP2 in green, and FAK in blue. C Effects of LAIR1^wt and LAIR1^Y251/281F mutant on LAIR1–SHP2 interaction by co-immunoprecipitation (n = 3). D FAK, p-FAK, cyclin D1, and CCL5 expressions in shLAIR1 GL261 cells expressing LAIR1^wt or LAIR1^Y251/281F constructs by Western blot and quantification (n = 4). E Immunofluorescence staining for FAK in shLAIR1 GL261 cells expressing LAIR1^wt or LAIR1^Y251/281F constructs (n = 3). F Colony formation abilities of shLAIR1 GL261 cells expressing LAIR1^wt or LAIR1^Y251/281F constructs by colony formation assay (n = 5). G Proliferation of shLAIR1 GL261 cells expressing LAIR1^wt or LAIR1^Y251/281F constructs by MTS (n = 5). H FAK, p-FAK, cyclin D1, and CCL5 expression in GL261 OE cells expressing SHP2^wt or SHP2^Q510E constructs by Western blot and quantification (n = 4). I Immunofluorescence staining for FAK in LAIR1OE GL261 cells expressing SHP2^wt or SHP2^Q510E constructs (n = 3). J Colony formation abilities of GL261 LAIR1OE cells expressing SHP2^wt or SHP2^Q510E constructs by colony formation assay (n = 5). K Proliferation of LAIR1OE GL261 cells expressing SHP2^wt or SHP2^Q510E constructs by MTS (n = 5). L Schematic of how LAIR1 drives glioma growth and macrophage immunosuppression. The data were expressed as average ±SD. P values were labeled in each figure.