Fig. 5: FOXQ1 activates the transcription of LDHA. | Cell Death & Disease

Fig. 5: FOXQ1 activates the transcription of LDHA.

From: FOXQ1 promotes pancreatic cancer cell proliferation, tumor stemness, invasion and metastasis through regulation of LDHA-mediated aerobic glycolysis

Fig. 5

A The intersection of pyruvate metabolism, glycolysis/gluconeogenesis, and differentially expressed genes (DEGs) in metabolic pathways is shown as a Venn diagram. B Correlation analysis of LDHA and FOXQ1 in PC using the GEPIA database. C Relative expression of LDHA in PC acquired from the GEPIA database. D Overall survival curve of PC patients with differential LDHA expression from the GEPIA database. E LDHA expression level was assessed in 96 pairs of PC (T) and corresponding adjacent (N) tissues by IHC assay. F Representative images of IHC assay for FOXQ1 and LDHA expression in successive slices of PC tissues. The expression of 40 samples was tested using Pearson’s chi-squared method. G and H Western blot (G) and RT-qPCR (H) analysis of LDHA expression in the indicated cells, respectively. I FOXQ1 binding motif predicted by TFDB. J Potential FOXQ1 binding locations in the LDHA promoter. K Diagrammatic representation of primers for LDHA areas. L Analysis of FOXQ1 enrichment on the LDHA promoter via ChIP-PCR. IgG served as the negative control. M Schematic of dual-luciferase reporter vectors. N Luciferase assays using the wild-type (Wt) and mutant (Mut) LDHA reporters in the indicated cells. Scale bar: 100 µm, *p < 0.05; **p < 0.01.

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