Fig. 7: LDHA is essential for FOXQ1-mediated PC cell invasion and metastasis in vitro. | Cell Death & Disease

Fig. 7: LDHA is essential for FOXQ1-mediated PC cell invasion and metastasis in vitro.

From: FOXQ1 promotes pancreatic cancer cell proliferation, tumor stemness, invasion and metastasis through regulation of LDHA-mediated aerobic glycolysis

Fig. 7

Small interfering RNA of LDHA (si-LDHA) and 2-DG (glycolytic pathway inhibitors) were added to PANC-1 and CFPAC-1 cells infected with lentivirus that overexpressed FOXQ1. A Wound healing assay of PANC-1 and CFPAC-1 cell groups. B, C Representative images of the Transwell assay in the indicated cells. D Immunofluorescence assay was performed to detect the EMT-related protein expression in PANC-1 and CFPAC-1 cell groups. E Western blotting experiment to detect the level of N-Cadherin, E-Cadherin, Vimentin, FOXQ1, and LDHA. Scale bar: 100 µm; *p < 0.05; **p < 0.01.

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