Fig. 2: USP39 extensively modulates alternative splicing in mice through potential interactions with RBPs.

A The overlapping DEGs in OE-NT vs. WT-NT and WT-T vs. WT-NT datasets (up, n = 382) and OE-T vs. WT-T and WT-T vs. WT-NT datasets (down, n = 24) were positively correlated. (Pearson Correlation test was performed.) B The GO biological process (BP) enrichment analysis of the overlapping DEGs in OE-NT vs. WT-NT and WT-T vs. WT-NT datasets. C The overlapping DAS events (n = 2833) were positively correlated in the OE-NT vs. WT-NT and WT-T vs. WT-NT datasets. D The GO biological process (BP) enrichment analysis of the overlapping DAS events in OE-NT vs. WT-NT and WT-T vs. WT-NT datasets. E and F RBP motif enrichment analyses within the four regions (5’ss1, 3’ss1, 5’ss2, 3’ss2) around the regulated cassette exons were compared with those around the background cassette exons. The start and end of each region were labeled by a nucleotide distance to the splice site. E Enriched motifs had a predominant distribution within the indicated regions around the regulated cassette exons compared with the background exons, while depleted motifs had less distribution around the regulated exons. The histogram described the enriched (left) or depleted (right) motifs in the indicated regions (F).