Fig. 5: HIF-1α shuttle induces CXCR4 modulation and CD133+ stem cells. | Cell Death & Disease

Fig. 5: HIF-1α shuttle induces CXCR4 modulation and CD133+ stem cells.

From: Extracellular vesicles from subjects with COPD modulate cancer initiating cells phenotype through HIF-1α shuttling

Fig. 5

A The increase of mRNA of CXCR4 in HBEC-KRASV12high induced by COPD-EVs was abrogated by the use of HIF-1α inhibitor. B The silencing of CXCR4 reversed the modulation of CD133+ CICs and CD133+CXCR4+ MICs in COPD-EVs treated. In all experiments, untreated cells were used as control. C The silencing of CXCR4 in HBEC-KRASV12high cells reversed the migratory capability gained by cells treated with COPD-EVs. D Pro-invasive properties given by the treatment with COPD-EVs to HBEC-KRASV12high cells were abolished by the silencing of CXCR4. E Colonies formation induced by COPD-EVs treatment in HBEC-KRASV12high cells was inhibited by CXCR4 silencing. *p < 0.05. Data are expressed as mean and SEM.

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