Fig. 3: Crystal structure of the p632/p732 hetero-tetramer in complex with DARPin F11. | Cell Death & Disease

Fig. 3: Crystal structure of the p632/p732 hetero-tetramer in complex with DARPin F11.

From: DARPins detect the formation of hetero-tetramers of p63 and p73 in epithelial tissues and in squamous cell carcinoma

Fig. 3

A The structure is shown in different orientations rotated by 90°. The DARPin is depicted in red, the p63 dimer in light blue and the p73 dimer in dark blue. B Detailed analysis of the interface of DARPin F11 with the hetero-tetramer showing the interacting side chains of the complex crystal structure analyzed by LigPlot. C Sequence of the DARPin F11 and alignment of OD sequences of p63 and p73 with amino acids highlighted involved in the interaction with the DARPin. Hydrogen bond contacts are highlighted in red, hydrophobic contacts in blue. The two mutations, E363K in the p73 OD and K377E in the p63 OD, that were introduced to stabilize the p632/p732 hetero-tetramer are marked in bold. D ITC results of a titration of the p632/p732 hetero-tetramer with the leucine zipper dimerized DARPin F11. The functional affinity (avidity) is too high to be measured by direct titration. No binding can be observed for the p63 and p73 homo-ODs.

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