Fig. 4: Potential molecular mechanisms involving NRSN2-AS1 and PTK2/β-catenin pathway in OC cells.

A, B An internal control was tubulin, western blotting was used to determine the protein level of PTK2, GSK3Y279/Y216, β-catenin, and β-cateninY142 in OC cells with silencing of NRSN2-AS1, n = 3. C Immunofluorescence experiments were performed to detect β-catenin expression levels (green) in the si-NRSN2-AS1 and si-PTK2 groups; DAPI was used to stain nuclei. Scale bar: 50 μm. D Quantification of fluorescence intensity from (C), n = 100 cells for each group. *P < 0.05, **P < 0.01, ***P < 0.001.