Fig. 4: Hepatocyte SIRT1 suppresses apoptosis and GSDME-mediated PCD in cold-stored mouse livers. | Cell Death & Disease

Fig. 4: Hepatocyte SIRT1 suppresses apoptosis and GSDME-mediated PCD in cold-stored mouse livers.

From: SIRT1 regulates hepatocyte programmed cell death via GSDME - IL18 axis in human and mouse liver transplantation

Fig. 4

A Groups of FLOX and hSIRT1KO livers cold-stored in UW solution were perfused with physiological saline (0.5 ml) via a portal vein-cuff to collect liver flush from supra-hepatic inferior vena cava (n = 3/group). B WB-assisted detection of SIRT1, Bcl-2, XIAP, Pro-Casp3, cCasp3, GSDME-FL, GSDME-N, Pro-IL18, IL18, and β-actin in cold-stored livers (left panel). Some targets were evaluated in the liver flush by WB (right panel). C qRT-PCR assisted detection of mRNA coding for Bcl-2, Mcl1, XIAP, TNFα, IL10, NLRP3, IL1β, and IL18. D The relative intensity of Bcl-2, XIAP, GSDME-F, and GSDME-N normalized with β-actin in cold-stressed liver (left panel). The relative intensity of IL18, cCasp3, and GSDME-N in the liver flush (right panel). Data shown are mean ± SEM. *p < 0.05 by Student’s t-test.

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