Fig. 7: IL18 suppresses hepatocyte SIRT1 and anti-apoptotic axis. | Cell Death & Disease

Fig. 7: IL18 suppresses hepatocyte SIRT1 and anti-apoptotic axis.

From: SIRT1 regulates hepatocyte programmed cell death via GSDME - IL18 axis in human and mouse liver transplantation

Fig. 7

Primary hepatocytes transfected with control or IL18Rβ siRNA were subjected to cold stress (0–6 h). A WB-assisted detection of IL18Rβ, SIRT1, Bcl-2, XIAP, and β-actin (upper panels). The relative ratio of SIRT1, Bcl-2, and XIAP (lower panels). B qRT-PCR-assisted detection of mRNA coding for IL18Rβ, Bcl-2, and XIAP. C Primary hepatocytes transfected with control siRNA or SIRT1 siRNA and/or IL18Rβ siRNA were subjected to cold stress (6 h). WB-assisted detection of SIRT1, IL18Rβ, Bcl-2, XIAP, and β-Actin (upper panels). The relative intensity of SIRT1, IL18Rβ, Bcl-2, and XIAP (lower panels). Data shown are mean ± SEM. *p < 0.05; **p < 0.01 by one-way ANOVA.

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