Fig. 5: DNAJA4 recruits PSMD2 to ubiquitinate MYH9 and further causes its degradation. | Cell Death & Disease

Fig. 5: DNAJA4 recruits PSMD2 to ubiquitinate MYH9 and further causes its degradation.

From: DNAJA4 suppresses epithelial-mesenchymal transition and metastasis in nasopharyngeal carcinoma via PSMD2-mediated MYH9 degradation

Fig. 5

A Co-IP followed by western blotting using anti-DNAJA4 or anti-MYH9 antibodies revealed the endogenous association of DNAJA4 and PSMD2, as well as MYH9 and PSMD2, in HONE1 and SUNE1 cells. B Immunofluorescence staining indicated the cellular localization of exogenous HA-DNAJA4 and endogenous PSMD2 in HONE1 and SUNE1 cells transfected with the HA-DNAJA4 plasmid, as well as the cellular localization of endogenous PSMD2 and MYH9. Scale bar, 50 μm. C The mRNA and protein levels of MYH9 in HONE1 and SUNE1 cells transfected with the PSMD2 overexpression or empty plasmid. D The mRNA and protein levels of MYH9 in HONE1 and SUNE1 cells transfected with the shNC or shPSMD2 plasmid. E The effect of CHX treatment and greyscale analysis of MYH9 protein levels in HONE1 cells transfected with the PSMD2 overexpression or empty plasmid. F MG132 and CQ treatment in HONE1 cells transfected with the PSMD2 overexpression or empty plasmid. G HONE1 and SUNE1 cells transfected with the indicated plasmids were subjected to denaturing-IP and the ubiquitination of MYH9 and the indicated proteins were exogenously detected. H HONE1 and SUNE1 cells transfected with the indicated plasmids or shRNA were subjected to denaturing-IP, and the ubiquitination of MYH9 and the indicated proteins was exogenously detected. The data in (C, D, E) are shown as the mean ± SD, and p-values were determined by Student’s t test (ns, not significant; *p < 0.05).

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