Fig. 2: USP4 promotes ESCC cell proliferation in vitro and in vivo. | Cell Death & Disease

Fig. 2: USP4 promotes ESCC cell proliferation in vitro and in vivo.

From: USP4 promotes the proliferation, migration, and invasion of esophageal squamous cell carcinoma by targeting TAK1

Fig. 2: USP4 promotes ESCC cell proliferation in vitro and in vivo.

A, B USP4 protein levels in KYSE150 and KYSE180 cells after transfection with lentiviral solution were determined by Western blotting. C, D Colony formation was detected by single-cell clone assay. Representative images are shown (left panel), and the results were analyzed statistically (right panel). E–H Cell proliferation of transfected KYSE150 and KYSE180 cell lines was measured by CCK-8 assays (E, F) and EdU incorporation (G, H). I Representative images of nude mice and tumors at day 27 after inoculation of KYSE150 cells transfected with shCtrl or shUSP4 lentiviruses and KYSE180 cells transfected with vector or oeUSP4 lentiviruses (n = 5). J Quantitative analysis of tumor volume with the time lapse from day 7 onwards. K Comparison of tumor weight in the control and experimental groups. *P < 0.05, **P < 0.01, ***P < 0.001.

Back to article page