Fig. 7: BRISC positively regulates NF-κB activation in LPS-stimulated KCs. | Cell Death & Disease

Fig. 7: BRISC positively regulates NF-κB activation in LPS-stimulated KCs.

From: BRISC is required for optimal activation of NF-κB in Kupffer cells induced by LPS and contributes to acute liver injury

Fig. 7

A WT and Abro1−/− KCs were stimulated with 100 ng/ml LPS for various times. Immunoblot analysis of the indicated target proteins. B WT and Brcc3−/− KCs were stimulated with 100 ng/ml LPS for various times, followed by immunoblot analysis of the indicated target proteins. C WT and Abro1−/− PMs, neutrophils, and BMDMs were stimulated with 100 ng/ml LPS for the indicated time points, followed by immunoblot analysis of the indicated target proteins. D WT and Abro1−/− KCs transduced with lentivirus expressing NF-κB-luciferase reporter gene were treated with 100 ng/ml LPS for 3 h. The luminescence levels were measured and normalized to control values. Nuclear and cytoplasmic proteins of WT and Abro1−/− KCs were extracted after stimulation with LPS for 30 min. E Immunoblot analysis of p65 expression in the cytoplasm and nucleus. F ELISA of the DNA binding activity of nuclear NF-κB p65. WT and Abro1−/− KCs were pre-treated with NF-κΒ activator 1 G or NF-κΒ activator 2 H for 6 h and then treated with 100 ng/ml LPS for 3 h. CBA analysis of TNF-α and IL-6. Data are presented as means ± SEM; **P < 0.01; ***P < 0.001; two-tailed unpaired t-test.

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