Fig. 4: Correlation of the molecular consequences of high MAFB and low MYB in AML patients with MLL-rearrangements.

A Violin plot representing the boundaries of MAFB^low (lower quartile, 0–25% of expression range) and MAFB^high (upper quantile, 75–100% of expression range) patients in the subgroups with complex karyotype (left) and MLL-rearrangements (right) from the Haferlach cohort. B Volcano plot indicating differentially expressed mRNAs with either negative (below -2 Log₂ FC, indicated as blue dots) or positive (above 2 Log₂ FC, indicated as red dots) correlation when comparing high versus low MAFB expressers in each AML subgroup. C Differential expression of representative MYB myeloid target genes (BCL2, CDK2, CDK6, GFI1, KIT, MEIS2, BCL6, CD14, DUSP6, ITGAM, S100A8, and S100A9) comparing low versus high expressers in both complex karyotype and MLL-rearrangements subgroups. Data are presented as overlapping scatter plots in which MAFB^high patients are indicated in red and MAFB^low patients are indicated in blue. Every plot shows a colour-coded boxplot showing a median interquartile range. The statistical analysis shown in each plot indicates p-value adjusted for false discovery rates (***< 0.001, **< 0.01, *< 0.05). D Euler diagrams indicating the numbers and overlap of genes that display negative correlation with MAFB and positive correlation with MYB (left panel) or genes that display positive correlation with MAFB and negative correlation with MYB (right panel) in the subgroup of patients carrying MLL-rearrangements.