Fig. 1: CircSKA3 characteristics and expression in CRC. | Cell Death & Disease

Fig. 1: CircSKA3 characteristics and expression in CRC.

From: Specific intracellular retention of circSKA3 promotes colorectal cancer metastasis by attenuating ubiquitination and degradation of SLUG

Fig. 1

A CircRNA-sequencing heatmap of 10 paired normal intestinal mucosa–adenoma/adenocarcinoma tissue samples. B Venn diagram of upregulated circRNAs in CRC, adenoma and serum exosomes from CRC patients in the GEO database with AUC > 0.9. E Schematic illustration of circSKA3 circularization from exon 4 of the SKA3 gene and Sanger sequencing of the BSJ site of circSKA3. C, D The head-to-tail splicing of circSKA3 was clarified in cDNA and gDNA of HCT116 cells by RT‒PCR with different primers and RNase R treatment. E The stability of circSKA3 and linear SKA3 in HCT116 was assessed by RNase R treatment followed by RT‒qPCR. F Evaluation of the stability of circSKA3 and SKA3 mRNA in HCT116 treated with actinomycin D. G Expression levels of circSKA3 in 55 paired normal and CRC samples as determined by RT‒qPCR (normalized to GAPDH). H K–M survival analysis of overall survival in CRC patients according to the circSKA3 expression level. I Detection of circSKA3 in the serum of 105 normal controls and 72 CRC patients. All results are representative of three independent experiments, and shown as mean ± SD. Statistical significance was accessed by paired-samples Student’s t-tests (G), Student’s t-test (E, F, I), and log-rank test (H). *p < 0.05, **p < 0.01, ***p < 0.001.

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