Fig. 2: CircSKA3 promotes CRC migration and invasion in vitro. | Cell Death & Disease

Fig. 2: CircSKA3 promotes CRC migration and invasion in vitro.

From: Specific intracellular retention of circSKA3 promotes colorectal cancer metastasis by attenuating ubiquitination and degradation of SLUG

Fig. 2

A Expression of circSKA3 in colorectal cell lines. B Schematic design of the shRNA sequence targeting the BSJ site of circSKA3. C Knockdown of circSKA3 via shRNA in HCT116 and SW620 cells. D Detection of the protein level of the host gene SKA3 after circSKA3 knockdown by immunoblotting. E Quantitative analysis of the migration and invasion abilities of HCT116 and SW620 cells after circSKA3 knockdown by Transwell assay. F Schematic of sgRNA design for the circSKA3 BSJ site. G Efficiency of circSKA3 knockdown by RfxCas13d-BSJ-gRNA in HCT116 and SW620 cells. H, I Transwell assay for the migration and invasion abilities of HCT116 and SW620 cells after circSKA3 knockdown by RfxCas13d-BSJ-gRNA. The right graph shows the quantitative analysis results (scale bar = 200 μm). J Effects of circSKA3-specific knockdown by RfxCas13d-BSJ-gRNA on HCT116 and SW620 cell proliferation. All results are representative of three independent experiments, and shown as mean ± SD (C, G, J) or mean ± SEM (A, E, H, I). Statistical significance was accessed by one-way ANOVA (A, C, E, G, H, I, J). ns, not significant with p > 0.05, **p < 0.01, ***p < 0.001.

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