Fig. 8: ZNF384 is an important transcription factor of Gαi1 in NPC cells.

The JASPAR database predicted the potential transcription factors of Gαi1 (A). pNPC-1 cells were transfected with the described siRNAs targeting different transcription factors or scramble non-sense siRNA (siC) for 48 h, expression of Gαi1 mRNA was examined (B). pNPC-1 cells with the lentiviral ZNF384 shRNA (shZNF384), the lentiviral CRISPR-ZNF384-KO construct (“koZNF384”), the scramble control shRNA plus CRISPR/Cas9 control construct (“shC+Cas9-C”), the lentiviral ZNF384-expressing construct (oeZNF384) or the empty vector (“Vec”) were established, expression of listed mRNAs and proteins was tested (C–F). Chromosome IP (ChIP) assay results showed the relative levels of ZNF384-bound Gαi1 promoter in the described NPC tumor tissues (“T”) and matched adjacent normal nasopharynx epithelial tissues (“N”) (G) as well as in the listed primary NPC cells and primary human nasal epithelial cells (HNEpC) (H). “Ctrl” stands the parental control cells. pNPC-1 cells with the lentiviral ZNF460 shRNA (“shZNF460”) or the scramble control shRNA (“shC”) were established, expression of listed proteins was tested (I). The expression of listed proteins in NPC tumor tissues (“T”, n = 20) and matched adjacent normal nasopharynx epithelial tissues (“N”, n = 20) was shown, with results quantified (J). The numerical values were mean ± standard deviation (SD). *P < 0.05 versus “siC” (B). *P < 0.05 versus “Ctrl”/“Vec” cells (C-F). *P < 0.05 versus “shC” (I).*P < 0.05 versus “N” tissues or pHNEpC-1 cells (G, H, J). Experiments in this figure were repeated five times, with similar results obtained.