Fig. 9: Gαi1 depletion inhibits NPC xenograft growth in nude mice.
From: Overexpressed Gαi1 exerts pro-tumorigenic activity in nasopharyngeal carcinoma
pNPC-1 xenograft-bearing nude mice were intratumorally injected Gαi1-shRNA-s2-expressing adeno-associated virus (“shGαi1-aav”) or scramble control shRNA adeno-associated virus (“shC-aav”), the volumes of pNPC-1 xenografts (A) and animal body weights (D) were recorded every six days. The estimated daily pNPC-1 xenograft growth was calculated (B); At day-42, all pNPC-1 xenografts were isolated and weights (C). The tissue lysates of the described pNPC-1 xenografts were obtained and expression of the described mRNAs and proteins was tested (E, F, G, H, and K), with Caspase-3 activity tested as well (J). Alternatively, the described pNPC-1 xenograft slices were subjected to IHC staining p-Akt (Ser-473) (I), or subjected to immuno-fluorescence staining of TUNEL/DAPI (L). Values were mean ± standard deviation (SD). In (A–D), ten mice were in each group (n = 10). For (E–L), five random tissue pieces in each xenograft were tested (n = 5). *P < 0.05 vs. “shC-aav” group. Scale bar = 100 μm.
