Fig. 4: Abnormal p63 expression changes iCCA transcriptome. | Cell Death & Disease

Fig. 4: Abnormal p63 expression changes iCCA transcriptome.

From: ΔNp63α facilitates proliferation and migration, and modulates the chromatin landscape in intrahepatic cholangiocarcinoma cells

Fig. 4

Significant differentially expressed genes (DEG) (|log2FC | > log2(1.2) and padj < 0.05) were identified by comparing CCLP1 (CCLP1_sh2 vs CCLP1_scr) and RBE (RBE_over vs RBE_vector) RNA-seq data. A Volcano plot of all detected genes using log2(FoldChange) as x-axis and-log10(p.adjust) as y-axis. Upregulated DEG were shown in red, downregulated DEG were shown in green, while not significant altered genes were shown as grey dots; the p63 gene in each group is marked with a circle. B Significantly enriched terms (pvalue < 0.05) generated by GSEA analysis in CCLP1 group and RBE group respectively. NES > 0 indicates activated terms, NES < 0 indicates suppressed terms. C Venn diagram shows the 1196 overlapped DEG from CCLP1_DEG and RBE_DEG. D GO analysis of overlapped DEG showed significantly enriched GO terms which were classified into three subgroups. E Chord diagrams display enriched GO terms and the expression pattern of associated genes. F PPI network with identified hub genes correlated with p63. G Heatmap of the hub genes expression level. H Correlation between p63 and hub genes was analyzed by Pearson correlation coefficient (r), p < 0.05 and r > 0.40 indicates strong positive correlation. I Survival analysis of hub genes in iCCA patients from NODE dataset (OEP001105), with the mean value as threshold of high-expression group and low-expression group. P-value was calculated using the log-rank test, p < 0.05 was considered as statistically significant.

Back to article page