Fig. 5: p63 binding remodels chromatin accessibility at its target sites and alters the chromatin landscape. | Cell Death & Disease

Fig. 5: p63 binding remodels chromatin accessibility at its target sites and alters the chromatin landscape.

From: ΔNp63α facilitates proliferation and migration, and modulates the chromatin landscape in intrahepatic cholangiocarcinoma cells

Fig. 5

A Venn diagram comparing identified p63 ChIP-seq peaks in CCLP1_scr and CCLP1_sh2, B Venn diagram comparing identified p63 ChIP-seq peaks in RBE_OE (RBE_over) and RBE_EV (RBE_vector). C Metaplot and heatmap of p63 ChIP-seq reads 3 kb upstream and 3 kb downstream of each TSS in CCLP1_scr and CCLP1_sh2, RBE_OE and RBE_EV, respectively. D Barplot showing the distance of p63 peak summits from the TSS in CCLP1_lost_p63BS (CCLP1_scr vs CCLP1_sh2) and RBE_gained_p63BS (RBE_OE vs RBE_EV). E Top enrichment identified by HOMER known motif analysis on 500-bp regions centred on p63 peak summits in CCLP1_lost_p63BS and RBE_gained_p63BS respectively. F Metaplot and heatmap displaying the ATAC-seq signals from −3 kb to 3 kb surrounding the lost p63 peak summits (CCLP1_scr vs CCLP1_sh2) in CCLP1_scr and CCLP1_sh2, and the gained p63 peak summits (RBE_OE vs RBE_EV) in RBE_EV and RBE_OE. G Pie chart showing the genome-wide distribution of lost ATAC-seq peaks (CCLP1_scr vs CCLP1_sh2) in CCLP1_scr, and gained ATAC-seq peaks (RBE_OE vs RBE_EV) in RBE_OE. H Metaplot displaying the ChIP-seq signal of histone marks H3K27ac, H3K27me3, H3K4me1 and H3K4me3, 3 kb flanking the TSS in CCLP1_scr, CCLP1_sh2, RBE_EV, and RBE_OE, respectively. I Metaplot and heatmap displaying the ChIP-seq signal of p63, H3K4me1 and H3K4me3; data were plotted for 5 kb flanking the lost p63 peak summits (CCLP1_scr vs CCLP1_sh2) > 10 kb away from TSS in CCLP1_scr and CCLP1_sh2, and the gained p63 peak summits (RBE_OE vs RBE_EV) > 10 kb away from TSS in RBE_EV and RBE_OE, respectively.

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