Fig. 1: The LINC01133 expression changed the most significantly after ferroptosis induction.

A The panc-1 treated with cystine depletion for 24 h and the control group in triplicates were used for the mRNA and lncRNA sequencing. A total of 1105 upregulated transcripts (red pots) and 699 downregulated transcripts (blue plots) were screened (|Fold Change| >2, p < 0.05). B LINC01133 expression in PANC-1 NC, PANC-1 Erastin, PANC-1 Noc, PANC-1 Noc C, and FR. C LINC01133 expression in panc 04. 03 NC, PANC-1 Erastin, PANC-1 Noc, PANC-1 Noc C, and FR. D LINC01133 expression in SW1990 NC, PANC-1 Erastin, PANC-1 Noc, PANC-1 Noc C, and FR. The NC group means the cell lines were cultured in a standard medium. The erastin group implies that the erastin for PANC-1 and PANC04.03 was 10 µM, and SW1900 was 2.5 µM for 24 h. The Noc group means the cell lines were treated with cystine starvation for 24 h. The NOC C group suggests that the three cell lines were treated with cystine starvation for 12 h, followed by 12-h standard culturing. FR means ferroptosis-resistant PANC-1 cultured in cystine-depleted DMEM for 24 h. One-way ANOVA was used for the statistical analysis. NS means p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. All experiments were repeated three times independently.