Fig. 4: The regulation of CEBPB on LINC01133.

A The overlapping gene among the differentially expressed RNA molecules from the sequencing, the predicted transcription factors by JSAPAR and PROMO. CEBPB mRNA level (B) and protein level (C) among PANC-1 NC, PANC-1 Erastin, PANC-1 Noc, PANC-1 Noc C, and PANC-1 FR. The change of LINC01133 expression after the CEBPB knockdown (D) or overexpression (E) in PANC-1. The change of LINC01133 expression after the CEBPB knockdown (F) or overexpression (G) in SW1990. H The change of LINC01133 expression after the CEBPB knockdown in PANC-1 FR. I The MDA level of PANC-1 FR treated with cystine starvation for 72 h after CEBPB knockdown. J The cell proliferation of PANC-1 FR treated with CEBPB knockdown and cyctine starvation. The NC group means the PANC-1 was cultured in a standard DMEM for 24 h. The erastin group implies that PANC-1 was treated with 10 µM erastin for 24 h. The Noc group means the cell line was treated with cystine starvation for 24 h. The Noc C group suggests that the cell line was treated with cystine starvation for 12 h, followed by 12-h standard culturing. FR means ferroptosis-resistant PANC-1 cultured in cystine-depleted DMEM for 24 h. Si NC means the control siRNA. Si1 and si2 are two effective siRNAs targeting CEBPB. EV means the empty plasmid. OE implies CEBPB overexpression. The Student t-test and one-way ANOVA were used for the statistical analysis. NS means p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. All experiments were repeated three times independently except A.