Fig. 5: The binding of CEBPB to the promotor LINC01133. | Cell Death & Disease

Fig. 5: The binding of CEBPB to the promotor LINC01133.

From: LINC01133 can induce acquired ferroptosis resistance by enhancing the FSP1 mRNA stability through forming the LINC01133-FUS-FSP1 complex

Fig. 5

A The predicted binding site of the LINC01133’s promoter with CEBPB and its location in the promoter. B The relative luciferase activity change in PANC-1 transfected with EV + pGL3, EV + pGL3-LINC01133 promoter, EV + pGL3-LINC01133 mutant promoter, CEBPB + pGL3, CEBPB + pGL3-LINC01133 promoter, and CEBPB + pGL3-LINC01133 mutant promoter. The overexpression detection of CEBPB was detected by the western blot below. C The anti-RNA Polymerase II – GAPDH promoter binding served as the positive control. The enrichment of LINC01133 promoter by CEBPB in PANC-1 (D), PANC-1 FR (E), and CFPAC-1 (F). EV means the empty plasmid. The Student t-test and one-way ANOVA were used for the statistical analysis. NS means p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. All experiments were repeated three times independently.

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