Fig. 8: The locolization and phase separation of LINC01133, FSP1 and FUS. | Cell Death & Disease

Fig. 8: The locolization and phase separation of LINC01133, FSP1 and FUS.

From: LINC01133 can induce acquired ferroptosis resistance by enhancing the FSP1 mRNA stability through forming the LINC01133-FUS-FSP1 complex

Fig. 8

A The location of LINC01133 (Cy3 label), FSP1 mRNA (FAM label) and FUS protein (AF647) by FISH and IF in panc-1, panc-1 FR, and panc-1 FR with FUS knockdown. In the merge picture, AF647 was labeled magenta with LINC01133 and FSP1 the same color. B The image of the phase separation of LINC01133, FUS and FSP1 mRNA. 300 ng/μL LINC01133 or FSP1 mRNA was used. For the FUS, 16 μM final concentration was chosen. The reaction was in the normal saline supplemented with 12.5% Ficoll-400.

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