Fig. 3: MtDSBs activate the DNA-PKcs-mediated type I interferon response.

a Western blot analysis of endogenous cGAS and STING levels in HEK293T and HeLa cells. b Western blot analysis to determine the levels of pIRF3 and pHSPA8 in HEK293T harvested after expression of MTERF1-FokI WT or MT for the indicated times. c Western blot analysis of pDNA-PKcs, pHSPA8 and pIRF3 levels in lysates from HEK293T cells expressing TFAM-FokI WT for 36 h. d, e Western blot analysis of pDNA-PKcs, pHSPA8 and pIRF3 levels after expression of TFAM-FokI WT or MTERF1-FokI WT in HEK293T cells pretreated with 12.5 μM NU7026 and 3 μM AZD7648. f Analysis of IFNB1, OAS1 mRNA levels in cGAS KO HeLa cells expressing TFAM-FokI WT or MTERF1-FokI WT pretreated with or without AZD7648. Data are means ± SD of n = 4; one-way ANOVA with CI = 95% and Bonferroni’s post hoc test. g Up, Western blot to analyze the levels of pHSPA8 after transfection of indicated TFAM-FokI into HEK293T cells with VDAC knockdown by siRNA. Down, Western blot analysis of pHSPA8 in cells collected 36 h after expression of the indicated TFAM-FokI in HEK293T cells pretreated with 80 μM VBIT-12. h Western blot to analyze the levels of pHSPA8, pIRF3 and pDNA-PKcs 36 h after transfection with the indicated TFAM-FokI in HEK293T cells transfected with siBAX and siBAK. Each experiment was performed at least three times. **P ≤ 0.01, ***P ≤ 0.005.