Fig. 3: Human frataxin interacts with respiratory chain complexes I, II and III in healthy LCLs. | Cell Death & Disease

Fig. 3: Human frataxin interacts with respiratory chain complexes I, II and III in healthy LCLs.

From: Human frataxin, the Friedreich ataxia deficient protein, interacts with mitochondrial respiratory chain

Fig. 3: Human frataxin interacts with respiratory chain complexes I, II and III in healthy LCLs.The alternative text for this image may have been generated using AI.

A Representative confocal images of in situ PLA experiments performed as described in the legend of Fig. 1. B Western blotting analysis of co-immunoprecipitation (co-IP) experiments (representative image of one of three independent experiments). Input corresponds to the total cell lysate used for co-IP assay; flow through and final wash represent the fractions eluted from the incubation of cell lysate with the resin and collected after the third wash, respectively. The supernatant containing the IP sample after denaturation of the resin was loaded in the fourth lane. Equal volumes (i.e., 35 μL) of samples were loaded in each lane. Blots were immunodecorated with antibodies against FXN, NDUFS1 (complex I), SDHB (complex II) and UQCRFS1 (complex III).

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