Fig. 7: GABPB1 deficiency leads to mitochondrial dysfunction. | Cell Death & Disease

Fig. 7: GABPB1 deficiency leads to mitochondrial dysfunction.

From: HOMER3 promotes non-small cell lung cancer growth and metastasis primarily through GABPB1-mediated mitochondrial metabolism

Fig. 7: GABPB1 deficiency leads to mitochondrial dysfunction.

A Covariation between mRNA expression of GABPB1 (x axis) and indicated mitochondrial genes (y axis) in NSCLC in TCGA database. B, C The gene transfection efficiency was determined by Western blot analysis in A549 and H1299 cells. D, E The expression levels of genes related to “mitochondrial inner membrane” are determined by qRT-PCR analysis in A549 and H1299 cells. F Western blot analysis revealed that the levels of OPA1, UQCRC1, ATP5D, COX15 are reduced in H1299 cells treated with shGABPB1. G–J Measurements of the ECAR and OCR in A549 and H1299 cells treated with shGABPB1 treatment. K–N Effects of GABPB1 knockdown on the mitochondrial respiration capacity and glycolytic capacity in A549 and H1299 cells. O, P High-performance liquid chromatography (HPLC) analysis for ATP in A549 and H1299 cells (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001, NS, På 0.05, vs. the shControl group, one-way ANOVA followed by the Tukey’s post hoc test or two-tailed Student’s t-test.

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