Fig. 6: CUL4BAHR-mediated eEF2K ubiquitination. | Cell Death & Disease

Fig. 6: CUL4BAHR-mediated eEF2K ubiquitination.

From: The aryl hydrocarbon receptor controls mesenchymal stromal cell-mediated immunomodulation via ubiquitination of eukaryotic elongation factor-2 kinase

Fig. 6

A MSCs were stimulated with TNF-α, IFN-γ, and IL-1β. EEF2K proteins were immunoprecipitated and detected by an anti-Ub antibody. B Protein interaction between eEF2K and AHR was observed using an immunoprecipitation assay. C CUL4B proteins were immunoprecipitated, and their interaction with AHR was observed. D MSCs were stained with anti-AHR (green) and anti-eEF2K (red) antibodies, and the co-localization was measured by immunofluorescence. DAPI was used to count nuclear staining. E After cytokine stimulation, eEF2K protein levels were observed at the indicated time points. β-ACTIN was used as a loading control. All experiments were performed at least twice, and similar results were obtained.

Back to article page