Fig. 4: Apigenin modulates the splicing of genes involved in cell proliferation and apoptosis in TNBC cells. | Cell Death & Disease

Fig. 4: Apigenin modulates the splicing of genes involved in cell proliferation and apoptosis in TNBC cells.

From: Transcriptome reprogramming through alternative splicing triggered by apigenin drives cell death in triple-negative breast cancer

Fig. 4

A Enrichment analysis of tumor suppressors, oncogenes, and cancer drivers among the genes differentially spliced in cells treated with apigenin vs. DMSO was evaluated by a hypergeometric test. B Molecular functional enrichment analyses using IPA of genes differentially spliced in cells treated with apigenin. Statistical significance was evaluated by Fisher’s exact test. C–F Representative Sashimi plots (left) and PCR (right) validation of AS events in cell death-related genes in MDA-MB-231 and MCF10A cells treated with apigenin (Api) or DMSO control. A representative gel is shown at the bottom. Mean ± SEM of the PSI of the isoform indicated by the red arrow, n = 3. Statistical significance was evaluated by two-tail t-test.

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