Fig. 5: Apigenin restores the splicing of genes dysregulated in TNBC.
A Volcano plots depict ΔPSI in TNBC vs. NBT mammary tissues. Blue and yellow dots represent AS excluded and included, respectively, in TNBC vs. NBT. B The ASIs were segregated as excluded (blue) or included (yellow) in TNBC as compared to NBT. Statistical significance was evaluated by χ2 test with a Bonferroni correction. C Ratio of the number of included/excluded ASIs in TNBC vs. NBT. A ratio higher than 1 implies that the number of included ASI is higher in TNBC. D Scatter plot of ΔPSI of events dysregulated in TNBC compared to the ΔPSI in apigenin (Api)-treated MDA-MB-231 cells. Red dots indicate events that are switched by apigenin towards non-tumor levels while black dots indicate the non-switched events. E Venn diagram intersection of AS events affected by apigenin (Api) in MDA-MB-231 cells and the events dysregulated in TNBC. Among the shared genes, those switched by apigenin towards non-tumor levels are indicated in red. F Proportion of ASIs in TNBC compared to the proportion of events switched by apigenin towards non-tumor levels. Statistical significance was evaluated by test of proportion with Bonferroni correction. G Molecular functional enrichment analyses using IPA of genes switched by apigenin to non-tumor levels. Statistical significance was evaluated by Fisher’s exact test.
