Fig. 6: Apigenin decreases tumor growth in vivo altering AS of genes involved in tumorigenesis. | Cell Death & Disease

Fig. 6: Apigenin decreases tumor growth in vivo altering AS of genes involved in tumorigenesis.

From: Transcriptome reprogramming through alternative splicing triggered by apigenin drives cell death in triple-negative breast cancer

Fig. 6

A Line plots represent the mammary tumor volume in mice bearing MDA-MB-231 xenografts treated with 25 mg/kg apigenin or vehicle for 28 days. Mean ± SEM, n = 12. Statistical significance was evaluated by two-way ANOVA. B Representative tumors at day 28. C Tumor sections were stained by immunohistochemistry using anti-Ki67 antibodies. Ki67 index was calculated using the formula Ki67-positive cells/Total cells. D Tumor sections were stained by TUNEL. TUNEL-positive cells were counted and represented as counts per field (cpf). Mean ± SEM, n = 12 and statistical significance was evaluated by two-tailed t-test (C, D). E–H Total RNA was isolated from tumors and the AS of key genes involved in cell death was evaluated by isoform-specific PCR and agarose gel electrophoresis. Bar plots represent the Mean ± SEM of the PSI of the isoform indicated in red. Mean ± SEM, n = 5. Statistical significance was evaluated by two-tailed t-test.

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