Fig. 2: Knockout of NEDD4 protects CRC cells from 5-FU-induced apoptosis. | Cell Death & Disease

Fig. 2: Knockout of NEDD4 protects CRC cells from 5-FU-induced apoptosis.

From: The E3 ubiquitin ligase NEDD4 regulates chemoresistance to 5-fluorouracil in colorectal cancer cells by altering JNK signalling

Fig. 2

A Western blot analysis for the confirmation of NEDD4 KO in LIM1215 cell line. B FACS-based apoptosis was performed on LIM1215 WT and NEDD4 KO treated with 5-FU (5, 25 and 50 µM) for 72 h. Percentage of cell death is represented as percent sub-G1 (n = 4). C 5-FU (5, 25 and 50 µM) treated WT and NEDD4 KO LIM1215 cells were analysed for cell cycle profile. All data are represented as mean ± SEM. Significance is determined by two-tailed t-test.

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