Fig. 3: Knockout of NEDD4 does not show cross-resistance to oxaliplatin and irinotecan-induced apoptosis.

FACS-based apoptosis was performed on LIM1215 WT and NEDD4 KO cells treated with or without chemotherapeutic drug A oxaliplatin (n = 4) (0.5, 1 and 2 µM) and B irinotecan (n = 3) (25 and 50 µM) for 72 h. Percentage of cell death is represented as percent sub-G1. C, D Oxaliplatin and irinotecan-treated WT and NEDD4 KO LIM1215 cells, respectively, were analysed for cell cycle profile. All data are represented as mean ± SEM.