Fig. 4: Loss of NEDD4 altered cell morphology and reduced cell proliferation. | Cell Death & Disease

Fig. 4: Loss of NEDD4 altered cell morphology and reduced cell proliferation.

From: The E3 ubiquitin ligase NEDD4 regulates chemoresistance to 5-fluorouracil in colorectal cancer cells by altering JNK signalling

Fig. 4: Loss of NEDD4 altered cell morphology and reduced cell proliferation.The alternative text for this image may have been generated using AI.

A NEDD4 knockout in CRC cells leads to altered morphology. Representative phase contrast micrographs of LIM1215 WT and NEDD4 KO cells are shown. B Cell proliferation was measured by MTS assay at 24, 48 and 72 h (n = 4). C Reduced cell growth in NEDD4 KO clones was analysed using trypan blue assay which was performed at 24 and 48 h (n = 3). D Defects in two NEDD4 KO clones were analysed using colony formation assay. E Graphical representation of number of colonies counted (n = 3). F Representative image of wound healing assay LIM1215 WT and NEDD4 KO cells. The wound was created after the cells reached 100% confluency, migration was assessed at T0 and 16 h post-wounding. Images were taken under the ×4 objective of the light microscope. G Quantification of wound closure is showed (n = 3). All data are represented as mean ± SEM.

Back to article page