Fig. 4: RNA-seq identifies IGFBP5 as a novel target for BRG1.
From: The chromatin remodeling protein BRG1 regulates HSC-myofibroblast differentiation and liver fibrosis
A–E Primary murine HSCs were transfected with siRNA targeting BRG1 or scrambled siRNA (SCR). RNA-seq was performed as described in Methods. (A) Principal component (PC) plot. (B) Volcano plot. (C) GO analysis. (D) Geneset enrichment analysis (GSEA) analysis. (E) Heatmap. F, G Primary human/murine HSCs were transfected with siRNA targeting BRG1 or scrambled siRNA (SCR). IGFBP5 expression levels were examined by qPCR and Western blotting. H IGFBP5 promoter constructs were transfected into LX-2 cells followed by treatment with TGF-β (2 ng/ml) for 24 h. Luciferase activities were normalized by GFP fluorescence and protein concentration. I ChIP assays were performed in primary murine HSCs or LX-2 cells treated with TGF-β. J Murine or human primary HSCs were transfected with indicated siRNAs followed by transduction with lentivirus carrying an IGFBP5 expression vector. Myofibroblast marker gene expression levels were examined by qPCR. Data are expressed as the means ± SD. *p < 0.05.
