Fig. 4: cGAS deficiency increases CREB expression and phosphorylation in HBE cells.
From: Airway epithelial cGAS inhibits LPS-induced acute lung injury through CREB signaling
A The heat map of the differentially expressed genes (DEGs) (top 20 up-regulated and downregulate genes) in control- or cGAS-siRNA HBE cells through RNA sequencing analysis. Blue indicates a relatively low expression and red indicates a relatively high expression. B The volcano plot of differentially expressed genes (DEGs) in control- or cGAS-siRNA HBE cells through RNA sequencing analysis. C RT-qPCR of CREB in HBE cells transfected with cGAS siRNA or control siRNA for 24 h and incubated with 100 μg/ml LPS for an additional 24 h (n = 3). Representative immunofluorescence images (D) and quantification of staining (E) of P-CREB (red) and nuclei (DAPI, blue) in HBE cells transfected with cGAS siRNA or control siRNA for 24 h and incubated with 100 μg/ml LPS for an additional 24 h. Scale bars, 10 μm. F HBE cells were transfected with cGAS siRNA or control siRNA for 24 h and were treated with LPS (100 μg/ml) for 24 h, and the cell lysates were collected for immunoblotting. G Representative lung immunofluorescence images of P-CREB (red), nuclei (DAPI, blue), and airway epithelial cells (marked with CC10, green) in mice. Scale bars, 100 μm. Two-way ANOVA with Sidaks multiple comparisons (C, E), ***p < 0.001, ****p < 0.0001. The data presented are one representative experiment of three independent experiments and shown as mean ± SEM.
