Fig. 3: Temporal changes of the splenic marginal zone in acute versus chronic LCMV infection. | Cell Death & Disease

Fig. 3: Temporal changes of the splenic marginal zone in acute versus chronic LCMV infection.

From: Differential kinetics of splenic CD169+ macrophage death is one underlying cause of virus infection fate regulation

Fig. 3

a Schematic representation of timepoints of spleen sampling in acute and chronic LCMV infected mice and of anti-CD8α or anti-NK1.1 treatments in chronically LCMV-infected C57BL/6J mice. Frequencies of splenic LCMV-infected (VL4+) CD169+ macrophages (b) and of total splenic CD169+ macrophages (c) obtained by flow cytometry at days 3 and 5 post-infection from acute and chronic LCMV-infected mice. Absolute numbers of LCMV-infected (VL4+) CD169+ macrophages (d) and of total CD169+ macrophages (e) from days 3 and 5 post acute and chronic LCMV-infected mice. Frequencies (f) and absolute numbers (g) of splenic CD169+ macrophages at day 5 post-chronic LCMV infection in untreated and antibody-treated (anti-CD8α or anti-NK1.1) mice. For each group and time-point the mean ± SEM is shown; significance is determined via unpaired two-tailed t-test; *p ≤ 0.05; **p ≤ 0.01; ****p ≤ 0.0001. Immunofluorescence of spleen sections at the indicated time points after LCMV-infection from untreated or anti-CD8α antibody (aCD8) treated mice. Spleen sections were stained for VL4 (LCMV NP; red) and CD3 (T cells; blue) (h) and for CD169 (CD169+ Macrophages; green), CD3 (T cells; blue) and B220 (B cells; red) (i). One representative section of 3 mice per group is shown. Images were taken at 20X magnification, the scale bar indicates 300 µm.

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