Fig. 5: Transcription factor c-Myc bound to the FAM83F promoter to regulate FAM83F expression. | Cell Death & Disease

Fig. 5: Transcription factor c-Myc bound to the FAM83F promoter to regulate FAM83F expression.

From: Upregulation of FAM83F by c-Myc promotes cervical cancer growth and aerobic glycolysis via Wnt/β-catenin signaling activation

Fig. 5

A The transcription factor c-Myc bound to the promoter of FAM83F, as predicted by the JASPAR and TCGA database. B Binding site of c-Myc and FAM83F promoter sequence was predicted by the JASPAR database. C Relative luciferase activity of the FAM83F promoter was detected using a dual-luciferase reporter assay. D, E c-Myc expression was detected using qPCR and western blotting in CC cell lines (HeLa, C-33A, CaSki, and SiHa) and HCerEpiC. F–I The expression of c-Myc and FAM83F upon c-Myc overexpression in HeLa cells. The data represent the mean ± SD of three independent experiments, and the level of significance was indicated by ****P < 0.0001, ***P < 0.001, **P < 0.01, *P < 0.05. ns no significance (p > 0.05).

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