Fig. 1: Generation of a traceable mouse model of osteosarcoma cells.
From: WWOX promotes osteosarcoma development via upregulation of Myc
A Schematic illustration of the experimental OS mouse model. B Genomic PCR of Cre negative (Cre−), Cre positive (Cre+ WT) and Double knock out (DKOTOM) mice. Labels in the left panel represent the primer pairs and in the right panel represent the expected band size. C Fluorescent microscope images for bones extracted from (1) Cre-negative mouse, (2) Cre+ WT mouse, (3) DKOTOM mouse. D Immunofluorescence images of frozen bone sections from Cre−, Cre+ WT, and DKOTOM mice. Red signal represents the endogenous tdTomato, blue signal represents the nuclear staining-hoechst. E Flow cytometry analysis of BM cells of 2-months (2m) old Cre−, Cre+ WT, and DKOTOM mice. 10.2 ± 2.3 represents the percentage of tdTomato-positive cells in the Cre+ WT and DKO BM compared to the Cre- mice of similar age. F Immunoblotting of p53, WWOX, p21, and tdTomato in the Cre+ WT and DKO BM cells before (0 min) and after (30, 120 min) of ionizing radiation exposure (IR-10Gy). (Quantification relative to Cre+ WT is represented under the blot). WT wildtype, DKO double knock out, BF bright field, RFP red fluorescent protein, BM bone marrow.
