Fig. 4: young BM (yBM)-derived cells exhibit tumorigenic and metastatic ability.
From: WWOX promotes osteosarcoma development via upregulation of Myc
A Summary graph of flow cytometry analysis of the percentage of tdTomato-positive cells in the genetically manipulated BM (DKO BM) and wild-type BM cells (Cre+ WT BM) at different ages (n = 3). B Colony formation assay of BM cells from young non-tumor bearing mice (yBM-DKO) (n = 9), BM cells from Cre negative mice (Cre-BM) (n = 3) and Cre+ WT mice (Cre+ WT BM) (n = 3). Giemsa-stained colonies-left panel, fluorescent microscope images of the colonies-right panel, 77.6 ± 11.6-represents the number of colonies in yBM-DKO. C OS tumors developed in immunocompromised mice after 3–4 months of Intratibial (IT)-injection of yBM cells. H&E staining of the developed OS tumors. Histological validation represents fibroblastic/osteoblastic OS-upper panel. Immunofluorescence staining of the developed tumors. Red signal represents the endogenous tdTomato, blue signal represents the nuclear staining-hoechst. D Bar graph representing the percentage of immunocompromised mice that developed OS following IT injection of yBM, tumor and control (Cre-BM). E Light microscope images, tdTomato immunofluorescence and H&E staining respectively representing lung metastasis in NOD/SCID mice after intravenous injection (IV) of yBM cells. F Bar graph representing the percentage of NOD/SCOD mice that developed lung metastasis after IV injection of yBM cells (collected from 1.5 and 6-months old mice).
