Fig. 6: TRIM21 mediates the ubiquitination and degradation of Sohlh2. | Cell Death & Disease

Fig. 6: TRIM21 mediates the ubiquitination and degradation of Sohlh2.

From: TRIM21-mediated Sohlh2 ubiquitination suppresses M2 macrophage polarization and progression of triple-negative breast cancer

Fig. 6

A THP-1 cells were treated with CHX (50 μg/ml) and MG132 (20 μM) either alone or in combination for 6 h, and Western blot assay showed that Sohlh2 degraded via the ubiquitinproteasome pathway. Mass spectrometry (B, C) and Co-IP assay (D, E) were performed to detect the interactive proteins with Sohlh2. F The co-localization of TRIM21 and Sohlh2 was determined by immunofluorescent analysis. qPCR assay (G) and Western blot assay (H) showed the expression of Sohlh2 in TRIM21 overexpressing THP-1 cells. I THP-1 cells were treated with CHX (50 μg/ml) for different times, and the effect of TRIM21 on the half-life of Sohlh2 was analyzed by Western blot. J THP-1 cells were transfected as indicated, and coimmunoprecipitation and Western blot analysis were performed to detect the role of TRIM21 on the ubiquitination of Sohlh2. Scale bar indicates 20 μm. The values indicate the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001.

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