Fig. 4: Infiltration of tumorigenic CAFs in the tumor microenvironment of AEG.

A Representative images of immunohistochemistry staining showing the protein expression of COL1A1 in AEG tumors. B UMAP plot for two cell clusters of CAFs. C Expression of marker genes to identify iCAF and myCAF. D Heatmap showing the differential expressed genes between iCAF and myCAF. E Distinct enrichment of signaling pathways between iCAF and myCAF. F Survival curves of patients stratified according to the expression of COL1A1 or signatures of two CAF subclusters. Survival was measured using the Kaplan-Meier method and P values were calculated using log-rank test.