Fig. 2: The super-enhancer (SE) lncRNA LINC02454 is associated with glioma cell TMZ sensitivity. | Cell Death & Disease

Fig. 2: The super-enhancer (SE) lncRNA LINC02454 is associated with glioma cell TMZ sensitivity.

From: Bivalent activity of super-enhancer RNA LINC02454 controls 3D chromatin structure and regulates glioma sensitivity to temozolomide

Fig. 2

A Two lncRNAs that changed consistently in both TMZ-treated U251 and U87 cells (TMZ_diff_lncRNA) were located at glioma-specific SEs. Expression of LINC02454 (B) and MSRBS-AS1 (C) in GBM (166 cases) and normal brain (1157 cases) tissues. Gene Expression data are from TCGA and GTEx databases, ***p < 0.001. D qRT-PCR analysis of LINC02454 RNA levels in TMZ-treated (1 mM, 72 h) and Ctrl cells. Data represent means ± S.E.M. of three independent experiments. ***p < 0.001, compared with Ctrl cells. E, F RNA-seq of read counts of LINC02454 in Ctrl U251 and U87 cells treated with TMZ (1 mM, 72 h). G Analysis of survival data from glioma patients based on high and low LINC02454 expression. H ATAC-seq and H3K27ac and BRD4 and MED1 ChIP of the LINC02454 SE in glioma cells and astrocytes (NHA).

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