Fig. 4: MAP4K interactome and the effect on RANGAP1 subcellular distribution. | Cell Death & Disease

Fig. 4: MAP4K interactome and the effect on RANGAP1 subcellular distribution.

From: Screens in aging-relevant human ALS-motor neurons identify MAP4Ks as therapeutic targets for the disease

Fig. 4: MAP4K interactome and the effect on RANGAP1 subcellular distribution.The alternative text for this image may have been generated using AI.

A Western-blotting analysis of proteins after proximity labeling in ALS1-hiMNs at 10 dpi. B Venn diagram of proteins from two batches of samples analyzed by Mass Spectrometry. With a cutoff of 2.5-fold enrichment, 443 proteins were common to these two sets of data, including proteins with no expression in control group (0 in ctrl), proteins with over 2.5-fold changes, and proteins with over 10-fold changes. C The top 5 KEGG pathways. D STRING analysis of association networks of proteins in the top 4 KEGG pathways. E Validation of protein associations in HEK293T cells by co-immunoprecipitations (co-IP) and western blots. F co-IP assays in HEK293T cells showing association of MINK1mt with RANGAP1 or RAN. G Confocal images showing subcellular distribution of RANGAP1 or RAN in ALS1-hiMNs at 52 dpi. Arrows indicate aggregated cytoplasmic RANGAP1 foci. Scale bar, 10 µm. H MINKmt improves nuclear/cytoplasmic (Nuc/Cyt) ratios of the indicated proteins in ALS1-hiMNs at 52 dpi (mean ± SEM; n = 30 neurons per group; ***p < 0.001, Student’s t-test). I MINKmt improves soma sizes of ALS1-hiMNs at 52 dpi (mean ± SEM; n = 30 neurons per group; ***p < 0.001, Student’s t-test).

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