Fig. 7: NEDD8-regulated mitophagy conferred Treg function and mitochondrial respiration. | Cell Death & Disease

Fig. 7: NEDD8-regulated mitophagy conferred Treg function and mitochondrial respiration.

From: Targeting NEDD8 suppresses surgical stress-facilitated metastasis of colon cancer via restraining regulatory T cells

Fig. 7

NEDD8+/+ and NEDD8−/− mice were randomly subjected to laparotomy surgery after the inoculation of MC38 cells (n = 6 per group). Tregs were isolated form metastatic pulmonary tumors on D3 post-surgery and were treated with mitophagy inducer CCCP, mitochondrial division inhibitor 1 (Mdivi-1) or vehicle (DMSO). A The ATPase activity of Tregs was evaluated. B The morphology changes of mitochondria in Tregs were observed by TEM. C CCK8 Assay was used to determine the cell viability of Tregs. D The intensity of various critical protein expression in Tregs were detected by flow cytometry. E, F The mitochondrial respiration was analyzed by Cell Seahorse Mito Stress assay. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

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