Fig. 6: Mitophagy is required for gE-mediated inhibition of IFN production. | Cell Death & Disease

Fig. 6: Mitophagy is required for gE-mediated inhibition of IFN production.

From: Varicella zoster virus glycoprotein E facilitates PINK1/Parkin-mediated mitophagy to evade STING and MAVS-mediated antiviral innate immunity

Fig. 6

A, B HeLa-shSCR (shSCR), HeLa-shPINK1 (shPINK1), PINK1-V5 transfected HeLa-shPINK1 cells (shPINK1 + PINK1) were transfected with EV, VZV gE, along with IFN-β or ISRE luciferase reporter plasmids. Cells were also co-transfected with STING-HA (A), MAVS-MYC (B) for 24 h. As indicated, the cells were treated with 0.5 μg/mL poly(dA:dT) or 20 μg/mL poly(I:C) for 6 h. Relative luciferase activity is shown (mean ± SD; n = 3). *p < 0.05; **p < 0.01; ***p < 0.001 vs. EV-transfected cells. C HeLa-shSCR and HeLa-shPINK1 cells were co-transfected with EV or VZV gE, STING-HA, IFN-β or ISRE luciferase reporter plasmids for 24 h; 10 μM diABZI was added to activate STING-mediated IFN-β or ISRE promotor. **p < 0.01; ***p < 0.001 vs. EV-transfected cells. D, E HeLa-shSCR or HeLa-shPINK1 cells were transfected with EV or VZV gE (red), along with IRF3-GFP plasmid (green). After 0.5 μg/mL poly(dA:dT) or 10 μM diABZI treatment, IRF3 nuclear translocation from cytoplasm was observed. For quantification of IRF3 nuclear localization, a minimum of one hundred cells per condition were counted in three independent experiments. Data represent the mean ± SD of three independent experiments. ***p < 0.001 vs. EV-transfected cells.

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